NSUN2-mediated m5C modification of EPYC contributed to GC cell growth and metastasis, which provided a novel regulatory axis for understanding the pathogenesis of GC.

Rutin mitigates THP-induced cardiotoxicity and enhances chemotherapeutic efficacy via the novel miR-129-1-3p/GRIN2D pathway. Our data show miR-129-1-3p to be an essential mediator, and suggest that the rutin-miR-129-1-3p-GRIN2D axis is a favorable target for improving the safety and effectiveness of breast cancer chemotherapy.

P=N/A, N=250, Recruiting, University of Minnesota | Trial primary completion date: Dec 2025 --> Dec 2026

P4, N=78, Completed, Johns Hopkins Bloomberg School of Public Health | Active, not recruiting --> Completed

Future research should prioritize pharmacokinetic characterization, investigation of combinatorial therapeutic strategies, and pathways toward clinical translation. Intracellular exposure appears to be shaped by two complementary axes: interference with 3'end polyadenylation and ENT1/ENT2-mediated uptake with ADA-catalyzed deamination.

Although apoptosis is eventually induced in TM3 cells, protective autophagy is also activated to mitigate the cytotoxic impact of the combination treatment. This finding may provide a reference for the development of safe therapeutic regimen for Leydig cell tumors.

P2, N=127, Recruiting, Second Affiliated Hospital of Soochow University | Not yet recruiting --> Recruiting

Collectively, COR inhibited MM progression by inducing ferroptosis through upregulating CLEC2. The online version contains supplementary material available at 10.1007/s10616-025-00886-5.

Mechanistic investigations included quantification of global m6A levels, analysis of PCNA messenger RNA (mRNA) stability via actinomycin D assay, and western blot for protein expression. Our findings demonstrate that STM2457 overcomes cisplatin resistance in OS by targeting the METTL3-m6A-PCNA axis, thereby disrupting a key DNA repair and cell survival pathway. Pharmacological inhibition of METTL3 represents a novel and promising epigenetic strategy for resensitizing chemoresistant OS, supporting its potential for future clinical development.

The in vivo efficacy was further supported by Ki-67 expression in tumor tissues. Taken together, our findings demonstrated that although the combination of Act D and resveratrol showed better efficacy in vitro than any of the single agent, the in vivo efficacy was not improved.

Actinomycin D treatment and RNase R assay were performed to examine the stability of circMCM7...Notably, overexpression of MCM7 partially rescued the inhibitory effects on proliferation and invasion, as well as the pro-apoptotic effects induced by circMCM7 knockdown. This study elucidates the oncogenic function of circMCM7 in hepatocellular carcinoma and confirms its regulatory roles in HCC cell proliferation, invasion, and apoptosis through modulation of MCM7 expression, suggesting circMCM7 as a potential therapeutic target for HCC treatment.